PT - JOURNAL ARTICLE AU - LIU, WAI MAN AU - SCOTT, KATHERINE ANN AU - DALGLEISH, ANGUS GEORGE TI - Supernatants of Tumours Treated with Chemotherapy Can Alter Tumour Growth and Development <em>In Vivo</em> DP - 2015 Mar 01 TA - Anticancer Research PG - 1499--1508 VI - 35 IP - 3 4099 - http://ar.iiarjournals.org/content/35/3/1499.short 4100 - http://ar.iiarjournals.org/content/35/3/1499.full SO - Anticancer Res2015 Mar 01; 35 AB - Background: Tumour-derived supernatants are comprised of bioactive substances that have the capacity to transform host systems rendering them more supportive of tumour growth. Certain chemotherapies are able to alter the make-up of these supernatants. Materials and Methods: We explored the effects that vaccination with supernatants derived from tumours may have on tumour growth in a BALB/c model. Results: A number of cytokines were detected in the supernatants capable of increasing B-cell lymphoma 2 (BCL2) protein expression in cancer cells; of note, significantly higher levels of granulocyte-macrophage colony stimulating factor (GM-CSF) were detected in chemotherapy-treated supernatants compared to controls. Vaccinating mice with supernatants from untreated tumours significantly impeded the growth of sub-cutaneous-implanted tumours. However, this anticancer effect was significantly diminished if the supernatants used were from cancer cells treated with gemcitabine. Conclusion: The study lends in vivo support to the idea that tumours produce bioactive components that can influence host biology and that certain chemotherapies can negate these.