TY - JOUR T1 - Verification of a Canine PSMA (FolH1) Antibody JF - Anticancer Research JO - Anticancer Res SP - 145 LP - 148 VL - 35 IS - 1 AU - SIEGFRIED WAGNER AU - DENISE MAIBAUM AU - ANDREAS PICH AU - INGO NOLTE AU - HUGO MURUA ESCOBAR Y1 - 2015/01/01 UR - http://ar.iiarjournals.org/content/35/1/145.abstract N2 - Background: Canine prostate cancer (PC) is a highly aggressive malignancy. However, in contrast to man, neither standard screening strategies nor curative therapeutic options exist for the companion animal. A prostate-specific membrane antigen (PSMA) screening as molecular marker akin to human PC is currently not available for dogs as data on specific canine PSMA detection are contradictory. Materials and Methods: To evaluate an antibody for specific canine PSMA detection by western blotting (WB), lysates of three canine prostatic cell lines (CT1258, DT08/40, DT08/46) were comparatively analyzed by WB and mass spectrometry (MS) to the human cell lines VCaP, LnCaP and PC-3. Results: MS analyses of the detected canine proteins confirmed cross reactivity of the antibody clone YPSMA-1 with canine PSMA. Conclusion: The MS analyses of the extracted canine protein bands proved that the YPSMA-1 clone is as well specific for canine PSMA in WB and, thus, represents a reliable tool for comparative PSMA studies. ER -