TY - JOUR T1 - Dendrofalconerol A Suppresses Migrating Cancer Cells <em>via</em> EMT and Integrin Proteins JF - Anticancer Research JO - Anticancer Res SP - 201 LP - 205 VL - 35 IS - 1 AU - PREMKAMOL PENGPAENG AU - BOONCHOO SRITULARAK AU - PITHI CHANVORACHOTE Y1 - 2015/01/01 UR - http://ar.iiarjournals.org/content/35/1/201.abstract N2 - Background/Aim: Enhanced cell motility is a hallmark of highly metastatic cancer cells. The anti-migratory activity of Dendrofalconerol A (DF-A), a pure bis(bibenzyls) isolated from the stem of Dendrobium falconeri (Orchidaceae) is reported in the present study. Materials and Methods: Cytotoxicity effects of DF-A on H460 lung cancer cells was determined by the MTT assay. We also investigated the mechanism of DF-A-mediated EMT and integrin proteins level by western blotting. Results: DF-A at concentrations of 0.5-5 μM significantly reduced the protein level of migrating cells in a dose-dependent manner. The expression of migration-related integrins, including integrin β1 and integrin α4 was significantly reduced in response to DF-A treatment. Also, DF-A was shown to suppress epithelial to mesenchymal transition (EMT), as indicated by cadherin switch from N- to E-cadherin and decrease of Snail, Slug and vimentin. Conclusion: This study revealed the potential of DF-A, an anti-metastasis agent and the underlying mechanism in this in vitro assay with H460 cells, which leads to the development of a novel anti-metastatic agent. ER -