PT  - JOURNAL ARTICLE
AU  - THILL, MARC
AU  - REICHERT, KATHRIN
AU  - WOESTE, ALENA
AU  - POLACK, STEPHAN
AU  - FISCHER, DOROTHEA
AU  - HOELLEN, FRIEDERIKE
AU  - RODY, ACHIM
AU  - FRIEDRICH, MICHAEL
AU  - KÖSTER, FRANK
TI  - Combined Treatment of Breast Cancer Cell Lines with Vitamin D and COX-2 Inhibitors
DP  - 2015 Feb 01
TA  - Anticancer Research
PG  - 1189--1195
VI  - 35
IP  - 2
4099  - http://ar.iiarjournals.org/content/35/2/1189.short
4100  - http://ar.iiarjournals.org/content/35/2/1189.full
SO  - Anticancer Res2015 Feb 01; 35
AB  - Background: Vitamin D is known for its anti-cancerogenous potential. Prostaglandin E2 (PGE2) is a proliferation and inflammation activating agent. The production of PGE2 is dependent on the activity of cyclooxygenase-2 (COX-2). A link between vitamin D and PGE2 metabolism was recently shown. Materials and Methods: In MDA-MB-231 and MCF-7 breast cancer cell lines we investigated the influence of calcitriol and the COX-2 inhibitor celecoxib regarding cell growth via MTT test, as well as on the protein and mRNA expression of COX-2 using western blot and qRT-PCR. Results: The proliferation of MCF-7 and MDA-MB-231 was inhibited by both calcitriol and the COX-2 inhibitor celecoxib and even stronger by their combination. Moreover, calcitriol inhibited the COX-2 protein expression in MDA-MB-231, as well as the COX-2 mRNA expression in both cell lines. Conclusion: The combination of calcitriol and celecoxib demonstrated a cooperative growth-inhibiting effect in breast cancer cell lines.