PT - JOURNAL ARTICLE AU - YUKIHIKO HIROSHIMA AU - YONG ZHANG AU - NAN ZHANG AU - FUMINARI UEHARA AU - ALI MAAWY AU - TAKASHI MURAKAMI AU - SUMIYUKI MII AU - MAKO YAMAMOTO AU - SHINJI MIWA AU - SHUYA YANO AU - MASASHI MOMIYAMA AU - RYUTARO MORI AU - RYUSEI MATSUYAMA AU - TAKASHI CHISHIMA AU - KUNIYA TANAKA AU - YASUSHI ICHIKAWA AU - MICHAEL BOUVET AU - ITARU ENDO AU - ROBERT M. HOFFMAN TI - Patient-derived Orthotopic Xenograft (PDOX) Nude Mouse Model of Soft-tissue Sarcoma More Closely Mimics the Patient Behavior in Contrast to the Subcutaneous Ectopic Model DP - 2015 Feb 01 TA - Anticancer Research PG - 697--701 VI - 35 IP - 2 4099 - http://ar.iiarjournals.org/content/35/2/697.short 4100 - http://ar.iiarjournals.org/content/35/2/697.full SO - Anticancer Res2015 Feb 01; 35 AB - Aim: Soft-tissue sarcomas are a group of rare mesenchymal carcinomas that include approximately 50 histological types, and account for 1% of all adult cancer cases. The yearly incidence of soft-tissue sarcomas in the USA is approximately 11,280 cases, with an overall mortality of 3,900 deaths per year. Materials and Methods: In this study, we established a patient-derived orthotopic xenograft (PDOX) from a patient with a soft-tissue sarcoma of the retroperitoneum in nude mice and compared it to a subcutaneous patient-derived model of the same tumor for histology. Results: In the PDOX model, a bulky tumor grew in the left retroperitoneum in the same manner as the patient's tumor. Upon histological examination, the majority of the PDOX tissue section comprised sarcomatous high-grade spindle cells of varying sizes, similar to the original patient tumor. In contrast, the majority of the subcutaneously-implanted tumor comprised round to oval cells. Conclusion: These results indicate that the PDOX recapitulated the histology of the original tumor more than the subcutaneous model.