RT Journal Article SR Electronic T1 Human Papillomavirus and Breast Cancer: No Evidence of Association in a Spanish Set of Cases JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 851 OP 856 VO 35 IS 2 A1 MARIA VERNET-TOMAS A1 MARISA MENA A1 LAIA ALEMANY A1 IGNACIO BRAVO A1 SILVIA DE SANJOSÉ A1 PAU NICOLAU A1 ALBA BERGUEIRO A1 JOSEP MARIA COROMINAS A1 SERGI SERRANO A1 RAMON CARRERAS A1 BELEN LLOVERAS YR 2015 UL http://ar.iiarjournals.org/content/35/2/851.abstract AB Background/Aim: Great controversy exists about the association between Human Papillomavirus (HPV) and breast tumors. The aim of this study was to explore the presence of HPV DNA in a large set of breast cancer cases. Materials and Methods: Techniques used followed the standards for an international retrospective survey of HPV-DNA genotyping, coordinated by our own group and the DDL Laboratories in Rijswijk, the Netherlands. Paraffin-embedded samples were used. SPF-10 broad-spectrum primers were applied, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse-line probe assay. Results: A total of 78 samples were included in the study, 2 of benign conditions and 76 carcinomas, including different histological subtypes. HPV was not present in any of the specimens studied irrespective of histology, hormonal status and stage of disease. Conclusion: Our data do not support the involvement of HPV in breast carcinogenesis as no evidence of its presence was found.