TY - JOUR T1 - Human Papillomavirus and Breast Cancer: No Evidence of Association in a Spanish Set of Cases JF - Anticancer Research JO - Anticancer Res SP - 851 LP - 856 VL - 35 IS - 2 AU - MARIA VERNET-TOMAS AU - MARISA MENA AU - LAIA ALEMANY AU - IGNACIO BRAVO AU - SILVIA DE SANJOSÉ AU - PAU NICOLAU AU - ALBA BERGUEIRO AU - JOSEP MARIA COROMINAS AU - SERGI SERRANO AU - RAMON CARRERAS AU - BELEN LLOVERAS Y1 - 2015/02/01 UR - http://ar.iiarjournals.org/content/35/2/851.abstract N2 - Background/Aim: Great controversy exists about the association between Human Papillomavirus (HPV) and breast tumors. The aim of this study was to explore the presence of HPV DNA in a large set of breast cancer cases. Materials and Methods: Techniques used followed the standards for an international retrospective survey of HPV-DNA genotyping, coordinated by our own group and the DDL Laboratories in Rijswijk, the Netherlands. Paraffin-embedded samples were used. SPF-10 broad-spectrum primers were applied, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse-line probe assay. Results: A total of 78 samples were included in the study, 2 of benign conditions and 76 carcinomas, including different histological subtypes. HPV was not present in any of the specimens studied irrespective of histology, hormonal status and stage of disease. Conclusion: Our data do not support the involvement of HPV in breast carcinogenesis as no evidence of its presence was found. ER -