RT Journal Article
SR Electronic
T1 Is Relative Quantification Dispensable for the Measurement of MicroRNAs as Serum Biomarkers in Germ Cell Tumors?
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 117
OP 121
VO 35
IS 1
A1 MEIKE SPIEKERMANN
A1 KLAUS-PETER DIECKMANN
A1 THOMAS BALKS
A1 JÖRN BULLERDIEK
A1 GAZANFER BELGE
YR 2015
UL http://ar.iiarjournals.org/content/35/1/117.abstract
AB Background: Classical biomarkers α-fetoprotein, β-human chorionic gonadotropin and lactate dehydrogenase (AFP, bHCG and LDH) are elevated in only 60% of all testicular germ cell tumor (TGCT) patients. microRNAs (miRNAs) are a novel class of useful biomarkers in cancer and miRNAs of the miR-371-3 cluster were proven to be valuable markers for TGCT patients. Materials and Methods: We compared the Ct and ΔCt values of miR-371-3 by real time PCR (qPCR) with and without 18S rRNA for normalization. Expression of miR-371a-3p, miR-372 and miR-373-3p was measured in 25 TGCTs, 4 non-TGCTs and 17 age-matched male controls. Results: A highly positive correlation between Ct and ΔCt values was found in all samples. The highest correlation was found for miR-371a-3p (R2: 0.956). Conclusion: Results show that qPCR can be used without endogenous control for analyzing miR-371-3 in the serum of patients with testicular cancer and male controls if the technical procedure is performed under controlled conditions.