RT Journal Article
SR Electronic
T1 Human Adipose Tissue-derived Mesenchymal Stem Cells Inhibit Melanoma Growth In Vitro and In Vivo
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 159
OP 168
VO 35
IS 1
A1 JIN-OK AHN
A1 YE-RIN COH
A1 HEE-WOO LEE
A1 IL-SEOB SHIN
A1 SUNG-KEUN KANG
A1 HWA-YOUNG YOUN
YR 2015
UL http://ar.iiarjournals.org/content/35/1/159.abstract
AB Background/Aim: The effects of adipose tissue-derived mesenchymal stem cells (AT-MSCs) on the growth of human malignancies, including melanoma, are controversial and the underlying mechanisms are not yet-well understood. The aim of the present study was to investigate the in vitro and in vivo anti-tumor effects of human AT-MSCs on human melanoma. Materials and Methods: The inhibitory effect of AT-MSC-conditioned medium (AT-MSC-CM) on the growth of A375SM and A375P (human melanoma) cells was evaluated using a cell viability assay. Cell-cycle arrest and apoptosis in melanoma cells were investigated by flow cytometry and western blot analysis. To evaluate the in vivo anti-tumor effect of AT-MSCs, CM-DiI-labeled AT-MSCs were circumtumorally injected in tumor-bearing athymic mice and tumor size was measured. Results: AT-MSC-CM inhibited melanoma growth by altering cell-cycle distribution and inducing apoptosis in vitro. AT-MSCs suppressed tumor growth in tumor-bearing athymic mice and fluorescence analysis showed that AT-MSCs migrated efficiently to tumor tissues. Conclusion: AT-MSCs inhibit the growth of melanoma suggesting promise as a novel therapeutic agent for melanoma.