RT Journal Article SR Electronic T1 Human Adipose Tissue-derived Mesenchymal Stem Cells Inhibit Melanoma Growth In Vitro and In Vivo JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 159 OP 168 VO 35 IS 1 A1 JIN-OK AHN A1 YE-RIN COH A1 HEE-WOO LEE A1 IL-SEOB SHIN A1 SUNG-KEUN KANG A1 HWA-YOUNG YOUN YR 2015 UL http://ar.iiarjournals.org/content/35/1/159.abstract AB Background/Aim: The effects of adipose tissue-derived mesenchymal stem cells (AT-MSCs) on the growth of human malignancies, including melanoma, are controversial and the underlying mechanisms are not yet-well understood. The aim of the present study was to investigate the in vitro and in vivo anti-tumor effects of human AT-MSCs on human melanoma. Materials and Methods: The inhibitory effect of AT-MSC-conditioned medium (AT-MSC-CM) on the growth of A375SM and A375P (human melanoma) cells was evaluated using a cell viability assay. Cell-cycle arrest and apoptosis in melanoma cells were investigated by flow cytometry and western blot analysis. To evaluate the in vivo anti-tumor effect of AT-MSCs, CM-DiI-labeled AT-MSCs were circumtumorally injected in tumor-bearing athymic mice and tumor size was measured. Results: AT-MSC-CM inhibited melanoma growth by altering cell-cycle distribution and inducing apoptosis in vitro. AT-MSCs suppressed tumor growth in tumor-bearing athymic mice and fluorescence analysis showed that AT-MSCs migrated efficiently to tumor tissues. Conclusion: AT-MSCs inhibit the growth of melanoma suggesting promise as a novel therapeutic agent for melanoma.