RT Journal Article
SR Electronic
T1 Deregulation of Glyceraldehyde-3-Phosphate Dehydrogenase Expression During Tumor Progression of Human Cutaneous Melanoma
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 439
OP 444
VO 35
IS 1
A1 DAVID RAMOS
A1 ANA PELLÍN-CARCELÉN
A1 JAIME AGUSTÍ
A1 AMELIA MURGUI
A1 ESPERANZA JORDÁ
A1 ANTONIO PELLÍN
A1 CARLOS MONTEAGUDO
YR 2015
UL http://ar.iiarjournals.org/content/35/1/439.abstract
AB Background/Aim: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a highly abundant housekeeping gene. GAPDH overexpression has been reported in diverse types of human cancers including cutaneous melanoma. Our goal was to quantify GAPDH mRNA and protein expression in the whole spectrum of primary and metastatic melanomas in the search for a specific role for this ubiquitous molecule during tumor progression. Materials and Methods: Intratumoral GAPDH mRNA expression was quantified by real-time PCR in 71 cases, including 29 primary melanomas and 42 metastatic cases. Relative expression levels in thin (≤1 mm) and thick (>1 mm) primary tumors and ‘in-transit’, lymph node and distant metastases were compared. Similarly, protein expression was investigated by means of immunohistochemistry. Specific exons of GAPDH were analyzed by DNA sequencing. Results: GAPDH mRNA expression was significantly up-regulated in thick melanomas when compared to primary thin melanomas. Similar differences were also encountered between metastatic melanomas when compared to lymph-node metastatic melanomas. Interestingly, GAPDH protein immunoexpression was higher in thick melanomas and distant metastases than in thin tumors and lymph node metastases, respectively. However, no specific point-mutations in GAPDH-specific exons were found in any patient. Conclusion: Deregulation of GAPDH during melanoma progression was demonstrated in our series by mRNA and protein expression studies.