RT Journal Article
SR Electronic
T1 STAT3 Activation and Infiltration of Eosinophil Granulocytes in Mycosis Fungoides
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 5277
OP 5286
VO 34
IS 10
A1 SIMON FREDHOLM
A1 LISE METTE R. GJERDRUM
A1 ANDREAS WILLERSLEV-OLSEN
A1 DAVID L. PETERSEN
A1 INGER Ø. NIELSEN
A1 CLAUDIA-S. KAUCZOK
A1 MARION WOBSER
A1 ULRIK RALFKIAER
A1 CHARLOTTE M. BONEFELD
A1 MARIUSZ A. WASIK
A1 THORBJØRN KREJSGAARD
A1 CARSTEN GEISLER
A1 ELISABETH RALFKIAER
A1 ROBERT GNIADECKI
A1 ANDERS WOETMANN
A1 NIELS ODUM
YR 2014
UL http://ar.iiarjournals.org/content/34/10/5277.abstract
AB Eosinophil granulocytes have been implicated in anticancer immunity but recent data indicate that eosinophils can also promote cancer. Herein, we studied eosinophils in skin lesions from 43 patients with mycosis fungoides (MF). The presence of eosinophils correlated with disease stage: 78% of patients with advanced disease displayed eosinophil infiltration, whereas this was only seen in 11% of patients with patches (p<0.01), and in 48% of those with plaque disease. Importantly, 72% of patients with positive staining for phospho-signal-transducer-and-activator-of-transcription (pY-STAT3) in malignant T-cells also stained positively for eosinophils, whereas this was only observed in 28% of pY-STAT3-negative patients (p<0.01). Notably, malignant T-cells expressed eosinophilic activation and trafficking factors: High-mobility group BOX-1 protein (HMGB1) and interleukin 5 (IL5). STAT3 siRNA profoundly inhibited IL5 but not HMGB1 expression. In conclusion, these data suggest that malignant T-cells orchestrate accumulation and activation of eosinophils supporting the notion of STAT3 being a putative target for therapy.