PT - JOURNAL ARTICLE AU - SIMON FREDHOLM AU - LISE METTE R. GJERDRUM AU - ANDREAS WILLERSLEV-OLSEN AU - DAVID L. PETERSEN AU - INGER Ø. NIELSEN AU - CLAUDIA-S. KAUCZOK AU - MARION WOBSER AU - ULRIK RALFKIAER AU - CHARLOTTE M. BONEFELD AU - MARIUSZ A. WASIK AU - THORBJØRN KREJSGAARD AU - CARSTEN GEISLER AU - ELISABETH RALFKIAER AU - ROBERT GNIADECKI AU - ANDERS WOETMANN AU - NIELS ODUM TI - STAT3 Activation and Infiltration of Eosinophil Granulocytes in Mycosis Fungoides DP - 2014 Oct 01 TA - Anticancer Research PG - 5277--5286 VI - 34 IP - 10 4099 - http://ar.iiarjournals.org/content/34/10/5277.short 4100 - http://ar.iiarjournals.org/content/34/10/5277.full SO - Anticancer Res2014 Oct 01; 34 AB - Eosinophil granulocytes have been implicated in anticancer immunity but recent data indicate that eosinophils can also promote cancer. Herein, we studied eosinophils in skin lesions from 43 patients with mycosis fungoides (MF). The presence of eosinophils correlated with disease stage: 78% of patients with advanced disease displayed eosinophil infiltration, whereas this was only seen in 11% of patients with patches (p<0.01), and in 48% of those with plaque disease. Importantly, 72% of patients with positive staining for phospho-signal-transducer-and-activator-of-transcription (pY-STAT3) in malignant T-cells also stained positively for eosinophils, whereas this was only observed in 28% of pY-STAT3-negative patients (p<0.01). Notably, malignant T-cells expressed eosinophilic activation and trafficking factors: High-mobility group BOX-1 protein (HMGB1) and interleukin 5 (IL5). STAT3 siRNA profoundly inhibited IL5 but not HMGB1 expression. In conclusion, these data suggest that malignant T-cells orchestrate accumulation and activation of eosinophils supporting the notion of STAT3 being a putative target for therapy.