@article {FREDHOLM5277, author = {SIMON FREDHOLM and LISE METTE R. GJERDRUM and ANDREAS WILLERSLEV-OLSEN and DAVID L. PETERSEN and INGER {\O}. NIELSEN and CLAUDIA-S. KAUCZOK and MARION WOBSER and ULRIK RALFKIAER and CHARLOTTE M. BONEFELD and MARIUSZ A. WASIK and THORBJ{\O}RN KREJSGAARD and CARSTEN GEISLER and ELISABETH RALFKIAER and ROBERT GNIADECKI and ANDERS WOETMANN and NIELS ODUM}, title = {STAT3 Activation and Infiltration of Eosinophil Granulocytes in Mycosis Fungoides}, volume = {34}, number = {10}, pages = {5277--5286}, year = {2014}, publisher = {International Institute of Anticancer Research}, abstract = {Eosinophil granulocytes have been implicated in anticancer immunity but recent data indicate that eosinophils can also promote cancer. Herein, we studied eosinophils in skin lesions from 43 patients with mycosis fungoides (MF). The presence of eosinophils correlated with disease stage: 78\% of patients with advanced disease displayed eosinophil infiltration, whereas this was only seen in 11\% of patients with patches (p\<0.01), and in 48\% of those with plaque disease. Importantly, 72\% of patients with positive staining for phospho-signal-transducer-and-activator-of-transcription (pY-STAT3) in malignant T-cells also stained positively for eosinophils, whereas this was only observed in 28\% of pY-STAT3-negative patients (p\<0.01). Notably, malignant T-cells expressed eosinophilic activation and trafficking factors: High-mobility group BOX-1 protein (HMGB1) and interleukin 5 (IL5). STAT3 siRNA profoundly inhibited IL5 but not HMGB1 expression. In conclusion, these data suggest that malignant T-cells orchestrate accumulation and activation of eosinophils supporting the notion of STAT3 being a putative target for therapy.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/34/10/5277}, eprint = {https://ar.iiarjournals.org/content/34/10/5277.full.pdf}, journal = {Anticancer Research} }