RT Journal Article
SR Electronic
T1 Differential Expression of VEGF and IL-1alpha After Photodynamic Treatment in Combination with Doxorubicin or Taxotere
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 5295
OP 5302
VO 34
IS 10
A1 DAIVA DABKEVICIENE
A1 AUSRA SASNAUSKIENE
A1 EVA LEMAN
A1 RAIMONDA KVIETKAUSKAITE
A1 VIDA KIRVELIENE
YR 2014
UL http://ar.iiarjournals.org/content/34/10/5295.abstract
AB Aim: To show the impact of chemotherapeutic drugs doxorubicin and taxotere on the molecular pattern of cell response to photodynamic treatment (PDT). Materials and Methods: Human squamous cell carcinoma cells A-431 were studied. Apoptosis was investigated by recording caspase-3 activity. Expression of IL-1alpha and VEGF on mRNA and protein levels was measured by qPCR and ELISA. Results: PDT in combination with either doxorubicin or taxotere was found to be more cytotoxic in comparison to either single-treatment. The expression of IL-1alpha and VEGF was up-regulated in PDT-treated cells, either alone or in combination with doxorubicin or taxotere. Addition of doxorubicin to the cytokine induction after PDT was not detected, however, taxotere promoted significant over-expression of IL-1alpha and VEGF on the protein level. Conclusion: Contribution of chemotherapeutic drugs to IL-1 alpha and VEGF release from cells which received dual treatment involving PDT could be significantly different, despite the same level of cytotoxicity.