TY - JOUR T1 - Differential Expression of VEGF and IL-1alpha After Photodynamic Treatment in Combination with Doxorubicin or Taxotere JF - Anticancer Research JO - Anticancer Res SP - 5295 LP - 5302 VL - 34 IS - 10 AU - DAIVA DABKEVICIENE AU - AUSRA SASNAUSKIENE AU - EVA LEMAN AU - RAIMONDA KVIETKAUSKAITE AU - VIDA KIRVELIENE Y1 - 2014/10/01 UR - http://ar.iiarjournals.org/content/34/10/5295.abstract N2 - Aim: To show the impact of chemotherapeutic drugs doxorubicin and taxotere on the molecular pattern of cell response to photodynamic treatment (PDT). Materials and Methods: Human squamous cell carcinoma cells A-431 were studied. Apoptosis was investigated by recording caspase-3 activity. Expression of IL-1alpha and VEGF on mRNA and protein levels was measured by qPCR and ELISA. Results: PDT in combination with either doxorubicin or taxotere was found to be more cytotoxic in comparison to either single-treatment. The expression of IL-1alpha and VEGF was up-regulated in PDT-treated cells, either alone or in combination with doxorubicin or taxotere. Addition of doxorubicin to the cytokine induction after PDT was not detected, however, taxotere promoted significant over-expression of IL-1alpha and VEGF on the protein level. Conclusion: Contribution of chemotherapeutic drugs to IL-1 alpha and VEGF release from cells which received dual treatment involving PDT could be significantly different, despite the same level of cytotoxicity. ER -