TY - JOUR T1 - Antitumor Activity of Acriflavine in Lung Adenocarcinoma Cell Line A549 JF - Anticancer Research JO - Anticancer Res SP - 6467 LP - 6472 VL - 34 IS - 11 AU - CHIA-JEN LEE AU - CHIA-HERNG YUE AU - YU-JIE LIN AU - YU-YU LIN AU - SHAO-HSUAN KAO AU - JER-YUH LIU AU - YIENG-HOW CHEN Y1 - 2014/11/01 UR - http://ar.iiarjournals.org/content/34/11/6467.abstract N2 - Aim/Materials and Methods: In order to develop better drugs against non-small cell lung cancer (NSCLC), we screened a variety of compounds and treated the human lung adenocarcinoma cell line A549 with different drug concentrations. We then examined the cell viability using the MTT assay. Results: Data show that a new candidate drug, acriflavine (ACF), suppresses the viability of A549 cells in a concentration- and time-dependent manner. Flow cytometry analysis revealed that ACF significantly caused cell growth arrest in the G2/M phase on A549 cells. Moreover, ACF decreased Bcl-2 expression and increased Bax expression. The content of cleaved poly(ADP-ribose)polymerase-1 (PARP-1) and caspase-3 are significantly increased. These findings suggest that ACF is cytotoxic against A549 cells and suppresses A549 cells growth through the caspase-3 activation pathway. In the in vivo test, nude mice bearing A549 cells xenografts by intravenous injection were randomly assigned into two groups: control and experimental group. Treatment was initiated 10 days after implantation and intraperitoneal injection of 0.9% normal saline or 2 mg/kg of ACF was continued daily for five weeks. ACF treatment significantly decreased tumor size and tumor spots on lung surface of tumor-bearing mice. Conclusion: ACF can inhibit cell growth in A549 cells. Our results may assist on the delineation of the mechanism(s) leading to NSCLC cell growth inhibition and provide a new antitumor strategy against NSCLC. ER -