PT - JOURNAL ARTICLE AU - AGATA JARZĄB AU - ANETA GRABARSKA AU - MICHAŁ KIEŁBUS AU - WITOLD JELENIEWICZ AU - MAGDALENA DMOSZYŃSKA-GRANICZKA AU - KRYSTYNA SKALICKA-WOŹNIAK AU - ELWIRA SIENIAWSKA AU - KRZYSZTOF POLBERG AU - ANDRZEJ STEPULAK TI - Osthole Induces Apoptosis, Suppresses Cell-Cycle Progression and Proliferation of Cancer Cells DP - 2014 Nov 01 TA - Anticancer Research PG - 6473--6480 VI - 34 IP - 11 4099 - http://ar.iiarjournals.org/content/34/11/6473.short 4100 - http://ar.iiarjournals.org/content/34/11/6473.full SO - Anticancer Res2014 Nov 01; 34 AB - Background: The aim of the present study was to determine the effects of osthole on cell proliferation and viability, cell-cycle progression and induction of apoptosis in human laryngeal cancer RK33 and human medulloblastoma TE671 cell lines. Materials and Methods: Cell viability was measured by means of the MTT method and cell proliferation by the 5-bromo-2-deoxyuridine (BrdU) incorporation assay. Cell-cycle progression was determined by flow cytometry, and induction of apoptosis by release of oligonucleosomes to the cytosol. The gene expression was estimated by a quantitative polymerase chain reaction (qPCR) method. High-performance counter-current chromatography (HPCCC) was applied for isolation of osthole from fruits of Mutellina purpurea. Results: Osthole decreased proliferation and cell viability of cancer cells in a dose-dependent manner. The tested compound induced apoptosis, increased the cell numbers in G1 and decreased cell number in S/G2 phases of the cell cycle, differentially regulating CDKN1A and TP53 gene expression depending on cancer cell type. Conclusion: Osthole could be considered as a potential compound for cancer therapy and chemoprevention.