RT Journal Article
SR Electronic
T1 Protein and mRNA Expression of Notch Pathway Components in Operable Tumors of Patients with Laryngeal Cancer
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6495
OP 6503
VO 34
IS 11
A1 KRIKELIS, DIMITRIOS
A1 KOTOULA, VASSILIKI
A1 BOBOS, MATTHEOS
A1 FOUNTZILAS, ELENA
A1 MARKOU, KONSTANTINOS
A1 KARASMANIS, ILIAS
A1 ANGOURIDAKIS, NIKOLAOS
A1 VLACHTSIS, KONSTANTINOS
A1 KALOGERAS, KONSTANTINE T.
A1 NIKOLAOU, ANGELOS
A1 FOUNTZILAS, GEORGE
YR 2014
UL http://ar.iiarjournals.org/content/34/11/6495.abstract
AB Background: There exist substantial evidence that laryngeal cancer represents a unique entity among squamous head and neck carcinomas. Materials and Methods: Tumors from 289 patients with squamous cell laryngeal cancer were assessed for protein (immunohistochemistry) and mRNA (qRT-PCR) expression of Notch pathway components (Notch1 to 4 receptors and Jagged1 ligand) on tissue microarrays. Results: In univariate analysis, enhanced nuclear Jagged1 expression conferred a longer disease-free survival (DFS) (p=0.013) and overall survival (OS) (p=0.004), in contrast to the unfavorable prognostic value of Notch3 for both DFS (p=0.009) and OS (p=0.024). In multivariate analysis, overexpression of either Notch or cytoplasmic Jagged1 conferred an unfavorable effect on DFS (Hazard Ratio=1.88, 95% Confidence Interval=1.03-3.43, p=0.04). Conclusion: Our study indicates a consistent unfavorable effect of Notch3 and cytoplasmic Jagged1 protein expression, a favorable impact of nuclear Jagged 1 localization, and a differential prognostic value of Notch2 expression according to the presence of cytoplasmic Jagged 1.