PT  - JOURNAL ARTICLE
AU  - DIMITRIOS KRIKELIS
AU  - VASSILIKI KOTOULA
AU  - MATTHEOS BOBOS
AU  - ELENA FOUNTZILAS
AU  - KONSTANTINOS MARKOU
AU  - ILIAS KARASMANIS
AU  - NIKOLAOS ANGOURIDAKIS
AU  - KONSTANTINOS VLACHTSIS
AU  - KONSTANTINE T. KALOGERAS
AU  - ANGELOS NIKOLAOU
AU  - GEORGE FOUNTZILAS
TI  - Protein and mRNA Expression of Notch Pathway Components in Operable Tumors of Patients with Laryngeal Cancer
DP  - 2014 Nov 01
TA  - Anticancer Research
PG  - 6495--6503
VI  - 34
IP  - 11
4099  - http://ar.iiarjournals.org/content/34/11/6495.short
4100  - http://ar.iiarjournals.org/content/34/11/6495.full
SO  - Anticancer Res2014 Nov 01; 34
AB  - Background: There exist substantial evidence that laryngeal cancer represents a unique entity among squamous head and neck carcinomas. Materials and Methods: Tumors from 289 patients with squamous cell laryngeal cancer were assessed for protein (immunohistochemistry) and mRNA (qRT-PCR) expression of Notch pathway components (Notch1 to 4 receptors and Jagged1 ligand) on tissue microarrays. Results: In univariate analysis, enhanced nuclear Jagged1 expression conferred a longer disease-free survival (DFS) (p=0.013) and overall survival (OS) (p=0.004), in contrast to the unfavorable prognostic value of Notch3 for both DFS (p=0.009) and OS (p=0.024). In multivariate analysis, overexpression of either Notch or cytoplasmic Jagged1 conferred an unfavorable effect on DFS (Hazard Ratio=1.88, 95% Confidence Interval=1.03-3.43, p=0.04). Conclusion: Our study indicates a consistent unfavorable effect of Notch3 and cytoplasmic Jagged1 protein expression, a favorable impact of nuclear Jagged 1 localization, and a differential prognostic value of Notch2 expression according to the presence of cytoplasmic Jagged 1.