%0 Journal Article %A DIMITRIOS KRIKELIS %A VASSILIKI KOTOULA %A MATTHEOS BOBOS %A ELENA FOUNTZILAS %A KONSTANTINOS MARKOU %A ILIAS KARASMANIS %A NIKOLAOS ANGOURIDAKIS %A KONSTANTINOS VLACHTSIS %A KONSTANTINE T. KALOGERAS %A ANGELOS NIKOLAOU %A GEORGE FOUNTZILAS %T Protein and mRNA Expression of Notch Pathway Components in Operable Tumors of Patients with Laryngeal Cancer %D 2014 %J Anticancer Research %P 6495-6503 %V 34 %N 11 %X Background: There exist substantial evidence that laryngeal cancer represents a unique entity among squamous head and neck carcinomas. Materials and Methods: Tumors from 289 patients with squamous cell laryngeal cancer were assessed for protein (immunohistochemistry) and mRNA (qRT-PCR) expression of Notch pathway components (Notch1 to 4 receptors and Jagged1 ligand) on tissue microarrays. Results: In univariate analysis, enhanced nuclear Jagged1 expression conferred a longer disease-free survival (DFS) (p=0.013) and overall survival (OS) (p=0.004), in contrast to the unfavorable prognostic value of Notch3 for both DFS (p=0.009) and OS (p=0.024). In multivariate analysis, overexpression of either Notch or cytoplasmic Jagged1 conferred an unfavorable effect on DFS (Hazard Ratio=1.88, 95% Confidence Interval=1.03-3.43, p=0.04). Conclusion: Our study indicates a consistent unfavorable effect of Notch3 and cytoplasmic Jagged1 protein expression, a favorable impact of nuclear Jagged 1 localization, and a differential prognostic value of Notch2 expression according to the presence of cytoplasmic Jagged 1. %U https://ar.iiarjournals.org/content/anticanres/34/11/6495.full.pdf