RT Journal Article
SR Electronic
T1 Detection of Circulating Tumor Cells in Breast Cancer Patients Using Multiplex Reverse Transcription-Polymerase Chain Reaction and Specific Primers for MGB, PTHRP and KRT19 Correlation with Clinicopathological Features
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6691
OP 6699
VO 34
IS 11
A1 MARIA SKONDRA
A1 ELIONA GKIOKA
A1 IOANNIS D. KOSTAKIS
A1 NIKOLAOS PISSIMISSIS
A1 PANAGIOTIS LEMBESSIS
A1 DIMITRIOS PECTASIDES
A1 MICHAEL KOUTSILIERIS
YR 2014
UL http://ar.iiarjournals.org/content/34/11/6691.abstract
AB Aim: The aim of this study was to correlate the clinicopathological features of breast cancer patients with the positive detection of parathyroid hormone-related protein (PTHRP), cytokeratin protein 19 (KRT19) and mammaglobin (MGB) using a multiplex reverse transcription polymerase chain reaction (RT-PCR) assay developed to detect circulating tumor cells (CTCs) in peripheral blood of patients with breast cancer. Patients and Methods: Peripheral blood samples were collected from 54 breast cancer patients and 20 healthy blood donors. Subsequently, the samples were processed for RNA extraction and analyzed for the expression of PTHRP, KRT19 and MGB using specific primers and multiplex RT-PCR. Results: The positive detection rates in breast cancer patients for PTHRP, KRT19 and MGB were 68.5%, 63% and 22.2% and for healthy donors 10%, 0% and 10%, respectively. The statistical analysis revealed that PTHRP- and KRT19-positive detections correlated with the diagnosis of breast cancer while the combined positive detections of PTHRP-plus-KRT19 correlated with the presence of distant metastasis, especially with bone metastasis. Moreover, positive detections of KRT19 correlated with high proliferation rate of breast cancer tumors. MGB-positive detections did not add any diagnostic advantage in such analysis. Conclusion: Multiplex-PCR based detection of CTCs using PTHRP and KRT19 primers can provide useful information for the disease