PT  - JOURNAL ARTICLE
AU  - JACQUOT, CATHERINE
AU  - ROUSSEAU, BENEDICTE
AU  - CARBONNELLE, DELPHINE
AU  - CHINOU, IOANNA
AU  - MALLETER, MARINE
AU  - TOMASONI, CHRISTOPHE
AU  - ROUSSAKIS, CHRISTOS
TI  - Cucurbitacin-D-induced <em>CDK1</em> mRNA Up-regulation Causes Proliferation Arrest of a Non-small Cell Lung Carcinoma Cell Line (NSCLC-N6)
DP  - 2014 Sep 01
TA  - Anticancer Research
PG  - 4797--4806
VI  - 34
IP  - 9
4099  - http://ar.iiarjournals.org/content/34/9/4797.short
4100  - http://ar.iiarjournals.org/content/34/9/4797.full
SO  - Anticancer Res2014 Sep 01; 34
AB  - Despite progress in chemotherapeutic agents, non-small cell lung cancers (NSCLC) still have a poor survival rate. Thus, development of new therapeutic strategies, specifically against cancer cells is still required. For this purpose, we treated the non-small cell lung cancer cell line NSCLC-N6 with the natural product cucurbitacin D (CucD) - extracted from the plant Ecballium elaterium in order first to assess its in vitro cytotoxicity, but also to study the genetic changes that it could bring out. CucD has shown a blocking in the G1 phase of the cell cycle in NSCLC-N6 cells prior to apoptotic cell death. The reverse transcriptase-polymerase chain reaction-differential display (RT-PCR-DD) technique was also applied on treated cells to elucidate the genetic mechanisms involved. We revealed an overexpression of Cyclin-dependent kinase 1 (CDK1) mRNA after treatment and, with the use of antisense oligonucleotides, an effective role in the proliferation arrest of NSCLC-N6 cells. The present study provides new insights about the mechanisms of proliferation arrest in tumor cells and open new ways of treatment to target tumor growth.