<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">JACQUOT, CATHERINE</style></author><author><style face="normal" font="default" size="100%">ROUSSEAU, BENEDICTE</style></author><author><style face="normal" font="default" size="100%">CARBONNELLE, DELPHINE</style></author><author><style face="normal" font="default" size="100%">CHINOU, IOANNA</style></author><author><style face="normal" font="default" size="100%">MALLETER, MARINE</style></author><author><style face="normal" font="default" size="100%">TOMASONI, CHRISTOPHE</style></author><author><style face="normal" font="default" size="100%">ROUSSAKIS, CHRISTOS</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Cucurbitacin-D-induced &lt;em&gt;CDK1&lt;/em&gt; mRNA Up-regulation Causes Proliferation Arrest of a Non-small Cell Lung Carcinoma Cell Line (NSCLC-N6)</style></title><secondary-title><style face="normal" font="default" size="100%">Anticancer Research</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2014-09-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">4797-4806</style></pages><volume><style face="normal" font="default" size="100%">34</style></volume><issue><style face="normal" font="default" size="100%">9</style></issue><abstract><style  face="normal" font="default" size="100%">Despite progress in chemotherapeutic agents, non-small cell lung cancers (NSCLC) still have a poor survival rate. Thus, development of new therapeutic strategies, specifically against cancer cells is still required. For this purpose, we treated the non-small cell lung cancer cell line NSCLC-N6 with the natural product cucurbitacin D (CucD) - extracted from the plant Ecballium elaterium in order first to assess its in vitro cytotoxicity, but also to study the genetic changes that it could bring out. CucD has shown a blocking in the G1 phase of the cell cycle in NSCLC-N6 cells prior to apoptotic cell death. The reverse transcriptase-polymerase chain reaction-differential display (RT-PCR-DD) technique was also applied on treated cells to elucidate the genetic mechanisms involved. We revealed an overexpression of Cyclin-dependent kinase 1 (CDK1) mRNA after treatment and, with the use of antisense oligonucleotides, an effective role in the proliferation arrest of NSCLC-N6 cells. The present study provides new insights about the mechanisms of proliferation arrest in tumor cells and open new ways of treatment to target tumor growth.</style></abstract></record></records></xml>