RT Journal Article SR Electronic T1 Heat-shock Protein HSPB1 Attenuates MicroRNA miR-1 Expression Thereby Restoring Oncogenic Pathways in Prostate Cancer Cells JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 3475 OP 3480 VO 34 IS 7 A1 MATTHIAS B. STOPE A1 CHRISTIAN STENDER A1 TINA SCHUBERT A1 STEFANIE PETERS A1 MARTIN WEISS A1 PATRICK ZIEGLER A1 UWE ZIMMERMANN A1 REINHARD WALTHER A1 MARTIN BURCHARDT YR 2014 UL http://ar.iiarjournals.org/content/34/7/3475.abstract AB Background: Heat-shock proteins (HSPs) as well as microRNAs have been identified to orchestrate crucial mechanisms in prostate cancer (PCa) progression and treatment resistance. Due to cytoprotective properties of HSPB1 we analyzed molecular mechanisms of drug resistance in PCa cell culture systems, and notably found HSPB1 functionality linked to microRNA miR-1 activities. Materials and Methods: HSPB1 and miR-1 levels were genetically modified in PCa cell lines and alterations in molecular and cellular responses were assessed by quantitative reverse transcription/polymerase chain reaction, western blotting, and proliferation assays. Results: Our data provided for the first time evidence that HSPB1 regulates miR-1 expression, and subsequently restores oncogenic signaling pathways of androgen receptor (AR) and transforming growth factor β1 (TGFB1). Conclusion: Our data point towards HSPB1 and miR-1 involvement in development of castration-resistant PCa and therefore represent promising targets for anticancer therapy of advanced PCa.