PT - JOURNAL ARTICLE AU - HIROKI SAITO AU - SATOSHI ANDO AU - NAOYA MORISHITA AU - KYUNG-MI LEE AU - DANTE DATOR AU - DAVID DY AU - KATSUMI SHIGEMURA AU - ZAINAL ADHIM AU - KEN-ICHI NIBU AU - MASATO FUJISAWA AU - TOSHIRO SHIRAKAWA TI - A Combined Lymphokine-activated Killer (LAK) Cell Immunotherapy and Adenovirus-<em>p53</em> Gene Therapy for Head and Neck Squamous Cell Carcinoma DP - 2014 Jul 01 TA - Anticancer Research PG - 3365--3370 VI - 34 IP - 7 4099 - http://ar.iiarjournals.org/content/34/7/3365.short 4100 - http://ar.iiarjournals.org/content/34/7/3365.full SO - Anticancer Res2014 Jul 01; 34 AB - Background: The antitumor activity of lymphokine activated killer (LAK) cells immunotherapy is not always effective in all patients, especially when used alone. In this study, we investigated the in vitro antitumor activities of a combination of LAK immunotherapy and gene therapy employing an adenovirus carrying the p53 gene (Ad-p53) in human head and neck squamous cell carcinoma. Materials and Methods: The in vitro cytotoxicity of LAK cells was tested in H891 cells infected with or without Ad-p53, and the mRNA expression levels of natural killer group 2D ligands (UL16 binding protein (ULBP) 1 to 5) and tumor necrosis factor (TNF-α) in these cells were measured by real-time reverse transcription polymerase chain reaction. Results: Ad-p53 infection increased the cytotoxicity of LAK cells against H891 cells, and also increased the mRNA expression levels of the ULBPs in H891 cells and TNF-α in the LAK cells. Conclusion: The antitumor activities of LAK cells in H891 cells were enhanced by Ad-p53. Conclusion: The combinational therapy of LAK immunotherapy and Ad-p53 gene therapy may represent a new paradigm for the treatment of head and neck cancer.