RT Journal Article
SR Electronic
T1 Histological Groups of Human Postpubertal Testicular Germ Cell Tumours Harbour Different Genetic Alterations
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4005
OP 4012
VO 34
IS 8
A1 VLADUŠIĆ, TOMISLAV
A1 HRAŠĆAN, RENO
A1 KRUŠLIN, BOŽO
A1 PEĆINA-ŠLAUS, NIVES
A1 PERICA, KRISTINA
A1 BIĆANIĆ, ANAMARIJA
A1 VRHOVAC, IVANA
A1 GAMULIN, MARIJA
A1 FRANEKIĆ, JASNA
YR 2014
UL http://ar.iiarjournals.org/content/34/8/4005.abstract
AB Background: Testicular germ cell tumours are the most common malignancies in young males. Molecular biology studies of these tumours are often contradictory. Two histological groups, seminoma and non-seminoma, differ both morphologically and in malignant behaviour. Although a common cytogenetic feature is seen, namely the amplification of the 12p chromosomal region, the development mechanisms of less aggressive seminomas and more aggressive non-seminomas are unknown. Materials and Methods: Occurrence of structural genetic alterations was analyzed in 18 seminomas and 22 non-seminomas for genes involved in the malignant tumour phenotype: cadherin 1, Type 1, E-cadherin (Epithelial), CDH1; adenomatous polyposis coli, APC; NME/NM23 nucleoside diphosphate kinase 1, NME1; tumour protein P53, TP53; cyclin-dependent kinase inhibitor 2A, CDKN2A; retinoblastoma 1, RB1; RAD51 recombinase, RAD51; mutS homolog 2, MSH2; MutL homolog 1, MLH1; breast cancer 1, early onset, BRCA1; BCL2-Associated X Protein, BAX; ATP-Binding Cassette, Sub-Family G (WHITE), Member 2, ABCG2. Genetic alterations, loss of heterozygosity and microsatellite instability, were analyzed using restriction fragment or microsatellite repeat length polymorphisms. Results: A difference in genetic alteration occurrence between seminomas and non-seminomas was observed. Conclusion: Occurrence of genetic alterations correlates with clinical behaviour of these tumours and may indicate that such alterations could occur early in the development of seminomas and non-seminomas.