RT Journal Article SR Electronic T1 Resveratrol Induces Luminal Apoptosis of Human Colorectal Cancer HCT116 Cells in Three-dimensional Culture JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 4551 OP 4555 VO 34 IS 8 A1 TOSHIYUKI TSUNODA A1 SHUHEI ISHIKURA A1 KEIKO DOI A1 HIROSHI MATSUZAKI A1 YURI IWAIHARA A1 SENJI SHIRASAWA YR 2014 UL http://ar.iiarjournals.org/content/34/8/4551.abstract AB Background: We have previously reported the crucial roles of oncogenic Kirsten rat sarcoma viral oncogene homolog (KRAS) in inhibiting apoptosis and disrupting cell polarity via the regulation of phosphodiesterase 4 (PDE4) expression in human colorectal cancer HCT116 cells in three-dimensional cultures (3DC). Herein we evaluated the effects of resveratrol, a PDE4 inhibitor, on the luminal cavity formation and the induction of apoptosis in HCT116 cells. Materials and Methods: Apoptosis was detected by immunofluorescence using confocal laser scanning microscopy with an antibody against cleaved caspase-3 in HCT116 cells treated with or without resveratrol in a two-dimensional culture (2DC) or 3DC. Results: Resveratrol did not induce apoptosis of HCT116 cells in 2DC, whereas the number of apoptotic HCT116 cells increased after resveratrol treatment in 3DC, leading to formation of a luminal cavity. Conclusion: Resveratrol induced apoptosis of HCT116 cells in 3DC, resulting in the formation of a luminal cavity, probably by inhibiting PDE4 activity.