%0 Journal Article %A ELENA DOLDO %A GAETANA COSTANZA %A AMEDEO FERLOSIO %A DANIELA PASSERI %A SERGIO BERNARDINI %A MARIA GIOVANNA SCIOLI %A DONATELLA MAZZAGLIA %A SARA AGOSTINELLI %A DONATELLA DEL BUFALO %A BERNARD CZERNOBILSKY %A AUGUSTO ORLANDI %T CRBP-1 Expression in Ovarian Cancer: A Potential Therapeutic Target %D 2014 %J Anticancer Research %P 3303-3312 %V 34 %N 7 %X Background/Aim: Cellular retinol binding protein-1 regulates retinol bioavailability and contributes to cell differentiation maintenance, but its role in ovarian carcinogenesis remains uncertain. We investigated CRBP-1 expression in ovarian tumors and CRBP-1 signaling-regulated pathways. Materials and Methods: We performed immunohistochemistry, methylation-specific PCR, gene copy number analysis in ovarian tumors and proliferation/apoptosis evaluation, gene array, blot and real-time PCR in CRBP-1-transfected A2780 ovarian cancer cells. Results: CRBP-1 expression was reduced or absent in G2 and G3 ovarian carcinomas. CRBP-1 silencing in 60% of G2 and 66.7% of G3 carcinomas was due to CRBP-1 promoter methylation. A2780 CRBP-1-transfected cells showed increased retinol-induced apoptosis, retinoid-induced reduced clonogenicity and down-regulation of proliferation and transcription genes, including AKT1, AKT3, EGFR, FOS, JUN, STAT1 and STAT5A. Conclusion: CRBP-1 loss in G2/G3 ovarian carcinomas and increased apoptotic susceptibility to retinoids in CRBP-1-transfected-A2780 cells suggest CRBP-1 screening as a target to ensure efficacy of an adjuvant retinoid therapy. %U https://ar.iiarjournals.org/content/anticanres/34/7/3303.full.pdf