TY - JOUR T1 - Combination of Lipitor and Celebrex Inhibits Prostate Cancer VCaP Cells <em>In Vitro</em> and <em>In Vivo</em> JF - Anticancer Research JO - Anticancer Res SP - 3357 LP - 3363 VL - 34 IS - 7 AU - HUARONG HUANG AU - XIAO-XING CUI AU - SHAOHUA CHEN AU - SUSAN GOODIN AU - YUE LIU AU - YAN HE AU - DONGLI LI AU - HONG WANG AU - JEREMIAH VAN DOREN AU - ROBERT S. DIPAOLA AU - ALLAN H. CONNEY AU - XI ZHENG Y1 - 2014/07/01 UR - http://ar.iiarjournals.org/content/34/7/3357.abstract N2 - Background/Aim: Lipitor is a cholesterol-lowering drug and Celebrex is a Cyclooxygenase-2 inhibitor. We investigated the effects of Lipitor and Celebrex on human prostate cancer VCaP cells cultured in vitro and grown as orthotopic xenograft tumors in SCID mice. Materials and Methods: Apoptosis was measured by morphological assessment and caspase-3 assay. Nuclear factor-kappa B (NF-κB) activation was determined by luciferase reporter assay. B-cell lymphoma-2 (Bcl2) was measured by western blotting and immunohistochemistry. Orthotopic prostate tumors were monitored by the IVIS imaging system. Results: the combination of Lipitor and Celebrex had stronger effects on the growth and apoptosis of VCaP cells than did either drug alone. The combination more potently inhibited activation of NFκB and expression of Bcl2 than either drug alone. The growth of orthotopic VCaP prostate tumors was strongly inhibited by treatment with the drug combination. Conclusion: Administration of Lipitor and Celebrex in combination may be an effective strategy for inhibiting the growth of prostate cancer. ER -