%0 Journal Article %A SATOSHI UENO %A RYUTA YAMAZAKI %A TAKASHI IKEDA %A TAKASHI YAEGASHI %A TAKESHI MATSUZAKI %T Antitumor Effect of a Novel Phenanthroindolizidine Alkaloid Derivative Through Inhibition of Protein Synthesis %D 2014 %J Anticancer Research %P 3391-3397 %V 34 %N 7 %X Aim: The present study aimed to determine the antitumor efficacy of a new Phenanthroindolizidine alkaloid (PA) derivative, YPC-10157, and to elucidate its mechanism of action. Materials and Methods: The in vitro and in vivo antitumor activity of YPC-10157 was evaluated against several human cancer cell lines and mouse xenograft models, respectively. Cell apoptosis was determined by measuring caspase-3/7 activity. The effect on protein synthesis was assessed using an in vitro cell-free translation assay system. Results: In vitro, YPC-10157 exhibited marked cell growth inhibition and induced apoptosis. In vivo, YPC-10157 had a strong antitumor effect on xenograft models of human colon cancer and leukemia. Moreover, YPC-10157 and its derivatives inhibited protein synthesis and their inhibitory activity on protein synthesis significantly correlated regarding cell growth. Conclusion: YPC-10157 has promising antitumor effects and suggest that its cytotoxic mechanism might involve the inhibition of protein synthesis. %U https://ar.iiarjournals.org/content/anticanres/34/7/3391.full.pdf