RT Journal Article
SR Electronic
T1 Proteinase K-containing Lipid Nanoparticles for Therapeutic Delivery of siRNA LOR-1284
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3531
OP 3535
VO 34
IS 7
A1 DONG CHUL KIM
A1 YOUNG AH CHO
A1 HONG LI
A1 BRYANT C. YUNG
A1 ROBERT J. LEE
YR 2014
UL http://ar.iiarjournals.org/content/34/7/3531.abstract
AB Background: The objective of the present study was to develop an efficient delivery vehicle for siRNA LOR-1284 through incorporation of proteinase K (PrK) as a means of preventing siRNA degradation by serum nucleases. Lipid nanoparticle-PrK-siRNA (LN-PrK-siRNA) complexes were synthesized and characterized. Materials and Methods: siRNA complexed with PrK and liposomes composed of dimethyldioctadecyl ammonium bromide/cholesterol/Tween 80 (60:35:5 molar ratio) were investigated for down-regulation of R2 mRNA activity in KB human carcinoma cells. Results: Treatment with LN-PrK-siRNA (30:0.3:1 molar ratio) significantly reduced levels of R2 mRNA compared to siRNA-liposomes without PrK in serum-containing medium. LN-PrK-siRNA complexes showed increased stability in serum and reduced toxicity in KB cells relative to LN-siRNA complexes. Conclusion: LN-PrK-siRNA complexes are promising delivery vehicles for siRNA.