TY - JOUR T1 - Proteinase K-containing Lipid Nanoparticles for Therapeutic Delivery of siRNA LOR-1284 JF - Anticancer Research JO - Anticancer Res SP - 3531 LP - 3535 VL - 34 IS - 7 AU - DONG CHUL KIM AU - YOUNG AH CHO AU - HONG LI AU - BRYANT C. YUNG AU - ROBERT J. LEE Y1 - 2014/07/01 UR - http://ar.iiarjournals.org/content/34/7/3531.abstract N2 - Background: The objective of the present study was to develop an efficient delivery vehicle for siRNA LOR-1284 through incorporation of proteinase K (PrK) as a means of preventing siRNA degradation by serum nucleases. Lipid nanoparticle-PrK-siRNA (LN-PrK-siRNA) complexes were synthesized and characterized. Materials and Methods: siRNA complexed with PrK and liposomes composed of dimethyldioctadecyl ammonium bromide/cholesterol/Tween 80 (60:35:5 molar ratio) were investigated for down-regulation of R2 mRNA activity in KB human carcinoma cells. Results: Treatment with LN-PrK-siRNA (30:0.3:1 molar ratio) significantly reduced levels of R2 mRNA compared to siRNA-liposomes without PrK in serum-containing medium. LN-PrK-siRNA complexes showed increased stability in serum and reduced toxicity in KB cells relative to LN-siRNA complexes. Conclusion: LN-PrK-siRNA complexes are promising delivery vehicles for siRNA. ER -