RT Journal Article
SR Electronic
T1 Spontaneous <em>In Vitro</em> Senescence of Glioma Cells Confirmed by an Antibody Against IDH1<sup>R132H</sup>
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2859
OP 2867
VO 34
IS 6
A1 STOCZYNSKA-FIDELUS, EWELINA
A1 OCH, WALDEMAR
A1 RIESKE, PIOTR
A1 BIENKOWSKI, MICHAL
A1 BANASZCZYK, MATEUSZ
A1 WINIECKA-KLIMEK, MARTA
A1 ZIEBA, JOLANTA
A1 JANIK, KAROLINA
A1 ROSIAK, KAMILA
A1 TREDA, CEZARY
A1 STAWSKI, ROBERT
A1 RADOMIAK-ZALUSKA, ANNA
A1 PIASKOWSKI, SYLWESTER
YR 2014
UL http://ar.iiarjournals.org/content/34/6/2859.abstract
AB Background: We have recently suggested that glioblastoma cells become spontaneously senescent in cell culture conditions. The antibody specific against IDH1R132H offers the perfect opportunity to verify this hypothesis. Materials and Methods: We analyzed the features of senescence in 8 glioma cell cultures showing the IDH1R132H mutation based on combination of immunocytochemistry, enzymo-cytochemistry, BrdU incorporation assay and real-time microscopic observation. Results: We report that glioma cells showing the IDH1R132H mutation become rapidly and spontaneously senescent in vitro. Senescence was observed in both classical and novel serum-free cell culture conditions. Importantly, the senescent IDH1R132H-positive cells showed the expression of stemness marker (SOX2). Conclusion: In vitro senescence appeared to be the main reason of the difficulties in any kind culturing of glioma cells. 3D cell cultures prolonged the survival and in vitro proliferation of neoplastic IDH1R132H-positive cells, however, did not enhance the stabilization efficiency. Senescence of glioma cells is spontaneously triggered in vitro, which offers the opportunity of potential new therapeutic strategies based on this phenomenon.