TY - JOUR T1 - Cell-Cycle Analysis and Apoptosis-associated Proteins in Cervical Lesions of Brazilian Women JF - Anticancer Research JO - Anticancer Res SP - 2789 LP - 2796 VL - 34 IS - 6 AU - CRISTINE DOBO AU - CELINA TIZUKO FUJIYAMA OSHIMA AU - FLÁVIO DE OLIVEIRA LIMA AU - THIAGO SIMÃO GOMES AU - JOÃO NORBERTO STÁVALE AU - VÍTOR ARIAS AU - DANIEL ARAKI RIBEIRO AU - GUSTAVO R.A. FOCCHI Y1 - 2014/06/01 UR - http://ar.iiarjournals.org/content/34/6/2789.abstract N2 - Aim: The aim of the present study was to detect the relative expressions of p53, p21Waf1/Cip1, p27Kip1 Bcl-2 and cleaved caspase-3 in cervical lesion samples from Brazilian women by immunohistochemistry. Materials and Methods: A total of 230 cervical biopsies in paraffin-embedded blocks were studied: 43 were invasive squamous cell carcinomas (SCC), 52 carcinomas in situ/cervical intraepithelial neoplasias III (CIN III), 54 cervical intraepithelial neoplasias II (CIN II), 51 cervical intraepithelial neoplasias I (CIN I) and 30 non-neoplastic lesions (NN) with benign cellular changes. Results: Significant differences were observed in the p53 expression between the different groups: NN and CIN I (p=0.010); NN and CIN II (p<0.00001); CIN II and CIN III (p=0.02); CIN II and CIS (p=0.0220); CIN II and CEC (p=0.010). Regarding p21WAF1/Cip1, significant differences were observed between NN and CEC (p=0.001); CIN I and CEC (p=0.001); CIN II and CIN III (p=0,001); CIN II and CIS (p=0.0004) and CIN II and CEC (p<0.0001). For p27Kip1, significant differences were observed between NN and CIN I (p<0.00001); NN and CIN II (p<0.00001); NN and CIS (p=0.038); CIN I and CIN III (p=0.001); CIN I and CIS (p=0.009); CIN I and CEC (p=0.0001); CIN II and CIN III (p=0.0003); CIN II and CIS (p=0.002); CIN II and CEC (p< 0.00001). Bcl-2 and caspase-3 did not show remarkable differences between groups. Conclusion: p53, p21WAF1/CIP1, p27KIP1 appear to be involved in the course of carcinogenesis. Rare expression of Bcl-2 and cleaved caspase-3 suggests that these proteins probably do not participate in cervical apoptosis. ER -