RT Journal Article
SR Electronic
T1 Cell-Cycle Analysis and Apoptosis-associated Proteins in Cervical Lesions of Brazilian Women
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2789
OP 2796
VO 34
IS 6
A1 CRISTINE DOBO
A1 CELINA TIZUKO FUJIYAMA OSHIMA
A1 FLÁVIO DE OLIVEIRA LIMA
A1 THIAGO SIMÃO GOMES
A1 JOÃO NORBERTO STÁVALE
A1 VÍTOR ARIAS
A1 DANIEL ARAKI RIBEIRO
A1 GUSTAVO R.A. FOCCHI
YR 2014
UL http://ar.iiarjournals.org/content/34/6/2789.abstract
AB Aim: The aim of the present study was to detect the relative expressions of p53, p21Waf1/Cip1, p27Kip1 Bcl-2 and cleaved caspase-3 in cervical lesion samples from Brazilian women by immunohistochemistry. Materials and Methods: A total of 230 cervical biopsies in paraffin-embedded blocks were studied: 43 were invasive squamous cell carcinomas (SCC), 52 carcinomas in situ/cervical intraepithelial neoplasias III (CIN III), 54 cervical intraepithelial neoplasias II (CIN II), 51 cervical intraepithelial neoplasias I (CIN I) and 30 non-neoplastic lesions (NN) with benign cellular changes. Results: Significant differences were observed in the p53 expression between the different groups: NN and CIN I (p=0.010); NN and CIN II (p<0.00001); CIN II and CIN III (p=0.02); CIN II and CIS (p=0.0220); CIN II and CEC (p=0.010). Regarding p21WAF1/Cip1, significant differences were observed between NN and CEC (p=0.001); CIN I and CEC (p=0.001); CIN II and CIN III (p=0,001); CIN II and CIS (p=0.0004) and CIN II and CEC (p<0.0001). For p27Kip1, significant differences were observed between NN and CIN I (p<0.00001); NN and CIN II (p<0.00001); NN and CIS (p=0.038); CIN I and CIN III (p=0.001); CIN I and CIS (p=0.009); CIN I and CEC (p=0.0001); CIN II and CIN III (p=0.0003); CIN II and CIS (p=0.002); CIN II and CEC (p< 0.00001). Bcl-2 and caspase-3 did not show remarkable differences between groups. Conclusion: p53, p21WAF1/CIP1, p27KIP1 appear to be involved in the course of carcinogenesis. Rare expression of Bcl-2 and cleaved caspase-3 suggests that these proteins probably do not participate in cervical apoptosis.