@article {LAI1811, author = {CHENG-KUO LAI and YERRA KOTESWARA RAO and KAI-RUEI CHANG and CHENG-WEN LIN and HONG-LIN SU and CHIA-SHUO CHANG and CHIH-HO LAI and YEW-MIN TZENG}, title = {3,3{\textquoteright},4{\textquoteright}, 5{\textquoteright}-Tetramethoxychalcone Inhibits Human Oral Cancer Cell Proliferation and Migration via p53-mediated Mitochondrial-dependent Apoptosis}, volume = {34}, number = {4}, pages = {1811--1819}, year = {2014}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: The current study aimed to identify an attractive target against human oral squamous cell carcinoma (OSCC). Materials and Methods: The effect of 3,3{\textquoteright},4{\textquoteright},5{\textquoteright}-tetramethoxychalcone (TMC) on OSCC cell proliferation, cell-cycle phase distribution, expression of markers of cell apoptosis, and cell migration were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, western blot, and transwell migration assay, respectively. Results: Experimental results revealed that TMC inhibited the OSCC cell proliferation (fifty percent inhibitory concentrations range=1.0-4.5 μM) by inducing G2/M phase arrest of the cell cycle. TMC caused DNA double-strand breaks, and enhanced expression of caspase-3 and -9, poly (ADP-ribose) polymerase, cytochrome c, calpain-1 and -2, phosphorylation of histone H2AX, phosphorylation of checkpoint kinases 2, p53, BCL2-antagonist/killer and BCL2-associated {\texttimes} protein, while reducing the mitochondrial membrane potential, and expression of B-cell lymphoma-2. In addition, TMC reduced the migration potential of OSCC cells by attenuating the C-C chemokine ligand 5/C-C chemokine receptor type 5 axis. Conclusion: These data indicate that TMC may be considered an interesting target for further development of chemotherapeutic agents against oral cancer.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/34/4/1811}, eprint = {https://ar.iiarjournals.org/content/34/4/1811.full.pdf}, journal = {Anticancer Research} }