TY - JOUR T1 - The Addition of a Pregnenolone Pendant Group Enhances the Anticancer Properties of Titanocene Dichloride in a MCF-7 Xenograft Model JF - Anticancer Research JO - Anticancer Res SP - 1609 LP - 1615 VL - 34 IS - 4 AU - GLADIANY RAMOS AU - YALIZ LOPERENA AU - GIOVANNI ORTIZ AU - FIORELLA REYES AU - ADA SZETO AU - JOSE VERA AU - JAVIER VELEZ AU - JESSICA MORALES AU - DEBORAH MORRERO AU - LINNETTE CASTILLO AU - SURANGANI DHARMAWARDHANE AU - ENRIQUE MELENDEZ AU - A. VALANCE WASHINGTON Y1 - 2014/04/01 UR - http://ar.iiarjournals.org/content/34/4/1609.abstract N2 - Background/Aim: Titanocene dichloride held great promise as a chemotherapeutic compound in pre-clinical studies. However, subsequent clinical trials revealed hepatoxicity and nephrotoxicity, which limited its use in clinical applications. Therefore, we used steroid pendant groups to improve the targeting of titanocene in MCF-7 breast cancer cells, and demonstrated a 10-fold lower effective dose compared to titanocene in in vitro assays. The aim of the present study was to test the efficacy of a titanocene functionalized with pregnenolone (Ti-Preg) in an in vivo breast cancer model. Materials and Methods: Xenografts from the MCF7 breast cancer cell line were implanted into athymic nu/nu mice to evaluate the potential of Ti-Preg as an anti-breast cancer agent. Results: Ti-Preg demonstrated significant inhibition of MCF-7 tumor growth when compared to vehicle and to titanocene controls. Conclusion: Our findings demonstrate the potential of steroid pendent groups for targeting chemotherapeutics to steroid hormone-dependent cancer. ER -