RT Journal Article
SR Electronic
T1 Silicate Fiber-based 3D Cell Culture System for Anticancer Drug Screening
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 5301
OP 5309
VO 33
IS 12
A1 YOSHIE YAMAGUCHI
A1 DAWEI DENG
A1 YOSHINORI SATO
A1 YUNG-TE HOU
A1 RIE WATANABE
A1 KOHEI SASAKI
A1 MASAAKI KAWABE
A1 EIICHI HIRANO
A1 TETSUO MORINAGA
YR 2013
UL http://ar.iiarjournals.org/content/33/12/5301.abstract
AB Background: Three-dimensional (3D) in vitro cultures can recapitulate the physiological in vivo microenvironment. 3D Modeling techniques have been investigated and applied in anticancer drug screening. Materials and Methods: A silicate fiber scaffold was used for 3D cell cultures, and used to model the efficacy of anticancer drugs, such as mytomicin C and doxorubicin. Results: A unique 3D structure was observed in 13 human tumor cell lines on scaffold, and these cells exhibited higher drug resistance than cells in two-dimensional (2D) cultures. Furthermore, the production of lactate and expression of the nuclear factor-kappa B (NF-κB)-regulated genes B cell lymphoma-2 (BCL2), cyclooxygenase-2 (COX2), and vascular endothelial growth factor (VEGF) were higher in 3D cultures than in 2D cultures. Conclusion: These findings suggest that a 3D model using a silicate fiber scaffold can mimic features of cancer, and is also a suitable model for the evaluation of anticancer drugs in vitro.