RT Journal Article SR Electronic T1 Reliable Housekeeping Gene Combination for Quantitative PCR of Lymph Nodes in Patients with Prostate Cancer JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 5243 OP 5248 VO 33 IS 12 A1 IGOR TSAUR A1 MARKUS RENNINGER A1 JOERG HENNENLOTTER A1 ELSIE OPPERMANN A1 MARITA MUNZ A1 URSULA KUEHS A1 ARNULF STENZL A1 DAVID SCHILLING YR 2013 UL http://ar.iiarjournals.org/content/33/12/5243.abstract AB Background: To reliably compare the results of gene expression studies, the expression of the target gene should be normalized to the expression of a reference gene. For lymph node metastases of prostate cancer, no data on polymerase chain reaction (PCR) normalization have yet been reported. We aimed to determine the most reliable reference gene combination for this purpose in patients with prostate cancer. Materials and Methods: Ten histologically- positive and ten negative lymph nodes of patients with prostate cancer were analyzed respectively. Expression of six candidate reference genes was comparatively assessed with quantitative Real-time PCR. The most stably-expressed gene combination was determined with geNorm software version 3.4. Results: Hypoxanthine phosphoribosyltransferase-1 (HPRT1) and TATA box binding protein (TPB) were found to be the most stably expressed genes, with their combination having an expression stability value of M=0.17. Conclusion: Gene combination HPRT1 and TPB has the potential to be utilized for normalization in gene profiling assessment of metastatic and non-metastatic pelvic lymph node tissue from patients with prostate cancer.