RT Journal Article SR Electronic T1 Impact of Interleukin-10 on Phenotype and Gene Expression During Early Monocyte Differentiation into Dendritic Cells JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 4791 OP 4798 VO 33 IS 11 A1 ANNIKA M. SCHWARZ A1 URSULA BANNING-EICHENSEER A1 KERSTIN SEIDEL A1 CHRISTINE MAUZ-KÖRHOLZ A1 DIETER KÖRHOLZ A1 MARTIN S. STAEGE YR 2013 UL http://ar.iiarjournals.org/content/33/11/4791.abstract AB Background: Cancer is linked to defects in immunosurveillance. Vaccination studies using dendritic cells (DC) try to re-establish immune responses toward tumor cells. Tumor-derived products such as interleukin-10 (IL-10) have inhibitory effects on DC function, and tumor-bearing hosts exhibit a lower number of DCs, suggesting inhibitory effects of tumor-derived factors on the recruitment of precursor cells. Materials and Methods: We generated DCs in the presence and absence of IL-10. DCs were then characterized by flow cytometry and cDNA microarray analysis. Results: IL-10 interferes with differentiation of peripheral blood monocytes to DCs and induces cells with a distinct phenotype. Microarray analysis revealed that IL-10 exhibits inhibitory as well as stimulatory effects on the expression of several genes. Addition of IL-10 to the differentiation cocktail induces a sustained inhibitory effect on subsequent maturation stimuli. Conclusion: IL-10 inhibits DC function and redirects differentiation of DCs to cells with a different phenotype, thereby reducing the pool of potential DC precursors.