PT - JOURNAL ARTICLE AU - ANNIKA M. SCHWARZ AU - URSULA BANNING-EICHENSEER AU - KERSTIN SEIDEL AU - CHRISTINE MAUZ-KÖRHOLZ AU - DIETER KÖRHOLZ AU - MARTIN S. STAEGE TI - Impact of Interleukin-10 on Phenotype and Gene Expression During Early Monocyte Differentiation into Dendritic Cells DP - 2013 Nov 01 TA - Anticancer Research PG - 4791--4798 VI - 33 IP - 11 4099 - http://ar.iiarjournals.org/content/33/11/4791.short 4100 - http://ar.iiarjournals.org/content/33/11/4791.full SO - Anticancer Res2013 Nov 01; 33 AB - Background: Cancer is linked to defects in immunosurveillance. Vaccination studies using dendritic cells (DC) try to re-establish immune responses toward tumor cells. Tumor-derived products such as interleukin-10 (IL-10) have inhibitory effects on DC function, and tumor-bearing hosts exhibit a lower number of DCs, suggesting inhibitory effects of tumor-derived factors on the recruitment of precursor cells. Materials and Methods: We generated DCs in the presence and absence of IL-10. DCs were then characterized by flow cytometry and cDNA microarray analysis. Results: IL-10 interferes with differentiation of peripheral blood monocytes to DCs and induces cells with a distinct phenotype. Microarray analysis revealed that IL-10 exhibits inhibitory as well as stimulatory effects on the expression of several genes. Addition of IL-10 to the differentiation cocktail induces a sustained inhibitory effect on subsequent maturation stimuli. Conclusion: IL-10 inhibits DC function and redirects differentiation of DCs to cells with a different phenotype, thereby reducing the pool of potential DC precursors.