@article {SCHWARZ4791, author = {ANNIKA M. SCHWARZ and URSULA BANNING-EICHENSEER and KERSTIN SEIDEL and CHRISTINE MAUZ-K{\"O}RHOLZ and DIETER K{\"O}RHOLZ and MARTIN S. STAEGE}, title = {Impact of Interleukin-10 on Phenotype and Gene Expression During Early Monocyte Differentiation into Dendritic Cells}, volume = {33}, number = {11}, pages = {4791--4798}, year = {2013}, publisher = {International Institute of Anticancer Research}, abstract = {Background: Cancer is linked to defects in immunosurveillance. Vaccination studies using dendritic cells (DC) try to re-establish immune responses toward tumor cells. Tumor-derived products such as interleukin-10 (IL-10) have inhibitory effects on DC function, and tumor-bearing hosts exhibit a lower number of DCs, suggesting inhibitory effects of tumor-derived factors on the recruitment of precursor cells. Materials and Methods: We generated DCs in the presence and absence of IL-10. DCs were then characterized by flow cytometry and cDNA microarray analysis. Results: IL-10 interferes with differentiation of peripheral blood monocytes to DCs and induces cells with a distinct phenotype. Microarray analysis revealed that IL-10 exhibits inhibitory as well as stimulatory effects on the expression of several genes. Addition of IL-10 to the differentiation cocktail induces a sustained inhibitory effect on subsequent maturation stimuli. Conclusion: IL-10 inhibits DC function and redirects differentiation of DCs to cells with a different phenotype, thereby reducing the pool of potential DC precursors.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/33/11/4791}, eprint = {https://ar.iiarjournals.org/content/33/11/4791.full.pdf}, journal = {Anticancer Research} }