TY - JOUR T1 - Loss of 5-Hydroxymethylcytosine and TET2 in Oral Squamous Cell Carcinoma JF - Anticancer Research JO - Anticancer Res SP - 4325 LP - 4328 VL - 33 IS - 10 AU - FREDRIK JÄWERT AU - BENGT HASSÉUS AU - GÖRAN KJELLER AU - BENGT MAGNUSSON AU - LARS SAND AU - LENA LARSSON Y1 - 2013/10/01 UR - http://ar.iiarjournals.org/content/33/10/4325.abstract N2 - Aim: Epigenetic modifications, such as DNA methylation, are considered important in the regulation of target genes in cancer development. 5-Hydroxymethylcytosine (5hmC) was recently discovered to be related to the process of malignant transformation. The influence of DNA methylation in oral squamous cell carcinomas (OSCC) is not fully-understood. Therefore, the aim of the present study was to investigate the DNA methylation pattern in OSCC compared to healthy oral epithelium. Materials and Methods: Oral mucosal samples from patients with OSCC (n=15) and healthy mucosa (n=12) were analyzed using immunohistochemistry with antibodies against 5hmC, 5mC and ten-eleven-translocation-2 (TET2). Results: A significant decrease in 5hmC and TET2 expression was found in OSCC compared to healthy oral epithelium. In contrast, there was a significant increase in 5mC expression in OSCC compared to healthy epithelium. Conclusion: Our results indicate that loss of 5hmC is an epigenetic event of OSCC. ER -