RT Journal Article SR Electronic T1 7-Substituted Coumarins Inhibit Proliferation and Migration of Laryngeal Cancer Cells In Vitro JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 4347 OP 4356 VO 33 IS 10 A1 MICHAŁ KIEŁBUS A1 KRYSTYNA SKALICKA-WOŹNIAK A1 ANETA GRABARSKA A1 WITOLD JELENIEWICZ A1 MAGDALENA DMOSZYŃSKA-GRANICZKA A1 ANDREW MARSTON A1 KRZYSZTOF POLBERG A1 PIOTR GAWDA A1 JANUSZ KLATKA A1 ANDRZEJ STEPULAK YR 2013 UL http://ar.iiarjournals.org/content/33/10/4347.abstract AB Background: Coumarins are a large group of naturally-occurring compounds with a wide range of biological properties, including anticancer activity. 7-Substituted coumarins (umbelliferone, scoparone, and herniarin) were analyzed for their potential anticancer activity against laryngeal cancer cells (LCC). Materials and Methods: High-performance counter-current chromatography was applied for successful separation of umbelliferone from fruits of Heracleum leskowii. A two-phase solvent system composed of n-heptane-methanol-ethyl acetate-water (1:2:1:2, v/v/v) was successfully used. Cell proliferation was assessed after 48-72 h by means of MTT test, and tumor cell motility by a wound assay model. Measurement of cell death was estimated using enzyme-linked immunosorbent assay (ELISA), and cell-cycle analysis was performed by flow cytometry. Extracellular signal-regulated kinases-1/2 (ERK1/2) and AKT kinase activation status were analyzed by western blotting. Results: Umbelliferone, scoparone, and, to a lesser extent, herniarin reduced viability and migration of RK33 LCC in a dose-dependent manner. Scoparone and herniarin were found to induce apoptosis of LCC. None of the tested compounds influenced the ERK1/2 and AKT kinase activity, nor significantly affected cell-cycle progression in the LCC line studied. Conclusion: Our findings suggest the therapeutic potential of 7-substituted coumarins in the treatment of laryngeal cancer.