TY - JOUR T1 - 7-Substituted Coumarins Inhibit Proliferation and Migration of Laryngeal Cancer Cells <em>In Vitro</em> JF - Anticancer Research JO - Anticancer Res SP - 4347 LP - 4356 VL - 33 IS - 10 AU - MICHAŁ KIEŁBUS AU - KRYSTYNA SKALICKA-WOŹNIAK AU - ANETA GRABARSKA AU - WITOLD JELENIEWICZ AU - MAGDALENA DMOSZYŃSKA-GRANICZKA AU - ANDREW MARSTON AU - KRZYSZTOF POLBERG AU - PIOTR GAWDA AU - JANUSZ KLATKA AU - ANDRZEJ STEPULAK Y1 - 2013/10/01 UR - http://ar.iiarjournals.org/content/33/10/4347.abstract N2 - Background: Coumarins are a large group of naturally-occurring compounds with a wide range of biological properties, including anticancer activity. 7-Substituted coumarins (umbelliferone, scoparone, and herniarin) were analyzed for their potential anticancer activity against laryngeal cancer cells (LCC). Materials and Methods: High-performance counter-current chromatography was applied for successful separation of umbelliferone from fruits of Heracleum leskowii. A two-phase solvent system composed of n-heptane-methanol-ethyl acetate-water (1:2:1:2, v/v/v) was successfully used. Cell proliferation was assessed after 48-72 h by means of MTT test, and tumor cell motility by a wound assay model. Measurement of cell death was estimated using enzyme-linked immunosorbent assay (ELISA), and cell-cycle analysis was performed by flow cytometry. Extracellular signal-regulated kinases-1/2 (ERK1/2) and AKT kinase activation status were analyzed by western blotting. Results: Umbelliferone, scoparone, and, to a lesser extent, herniarin reduced viability and migration of RK33 LCC in a dose-dependent manner. Scoparone and herniarin were found to induce apoptosis of LCC. None of the tested compounds influenced the ERK1/2 and AKT kinase activity, nor significantly affected cell-cycle progression in the LCC line studied. Conclusion: Our findings suggest the therapeutic potential of 7-substituted coumarins in the treatment of laryngeal cancer. ER -