RT Journal Article SR Electronic T1 Eicosapentaenoic Acid Modifies Cytokine Activity and Inhibits Cell Proliferation in an Oesophageal Cancer Cell Line JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 4319 OP 4324 VO 33 IS 10 A1 HISAKO KUBOTA A1 HIDEO MATSUMOTO A1 MASAHARU HIGASHIDA A1 HARUAKI MURAKAMI A1 HIROSHI NAKASHIMA A1 YASUO OKA A1 HIDEO OKUMURA A1 MASAHIRO YAMAMURA A1 MASAHUMI NAKAMURA A1 TOSHIHIRO HIRAI YR 2013 UL http://ar.iiarjournals.org/content/33/10/4319.abstract AB Background/Aim: The present study investigated the effect of eicosapentaenoic acid (EPA) on nuclear factor-kappa B (NF-κB) activation, inflammatory interleukin-6 (IL-6) production, and cell proliferation in a human oesophageal carcinoma cell line (TE-1). Materials and Methods: Lipopolysaccharide (LPS)-induced IL-6 production in TE-1 cells in the presence or absence of EPA was determined using enzyme-linked immunosorbent assay (ELISA). The proliferation of TE-1 cells was determined by the WST-1 assay. TE-1 cells were stained with Hoechst 33342 and propidium iodide to observe apoptosis. Immunohistochemical staining of NF-κB in TE-1 cells was performed. Results: LPS increased IL-6 production in TE-1 cells, and EPA treatment prevented this effect. EPA treatment inhibited NF-κB activation and induced apoptosis of TE-1 cells. Conclusion: EPA inhibits NF-κB activation and IL-6 production in oesophageal cancer cells, their inducing apoptosis. These effects of EPA may be of benefit in improving the outcome of cancer surgery.