TY - JOUR T1 - Multiple Anticancer Effects of Damsin and Coronopilin Isolated from <em>Ambrosia arborescens</em> on Cell Cultures JF - Anticancer Research JO - Anticancer Res SP - 3799 LP - 3805 VL - 33 IS - 9 AU - RODRIGO VILLAGOMEZ AU - GLORIA C. RODRIGO AU - ISIDRO G. COLLADO AU - MARCO A. CALZADO AU - EDUARDO MUÑOZ AU - BJÖRN ÅKESSON AU - OLOV STERNER AU - GIOVANNA R. ALMANZA AU - RUI-DONG DUAN Y1 - 2013/09/01 UR - http://ar.iiarjournals.org/content/33/9/3799.abstract N2 - Terpenoids in plants are important sources for drug discovery. In this study, we extracted damsin and coronopilin, two sesquiterpene lactones, from Ambrosia arborescens and examined their anticancer effects on cell cultures. Damsin and coronopilin inhibited cell proliferation, DNA biosynthesis and formation of cytoplasmic DNA histone complexes in Caco-2 cells, with damsin being more potent than coronopilin. Further studies using the luciferase reporter system showed that damsin and coronopilin also inhibited expressions of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription-3 (STAT3), indicating that these sesquiterpenes can interfere with NF-κB and STAT3 pathways. Finally, we examined the effects of two synthetic dibrominated derivatives of damsin, 11α,13-dibromodamsin and 11β,13-dibromodamsin. While bromination appeared to weaken the antiproliferative effects of damsin, the β epimer had strong inhibitory effects on STAT3 activation. In conclusion, the sesquiterpene lactones damsin and coronopilin have inhibitory effects on cell proliferation, DNA biosynthesis and NF-κB and STAT3 pathways, thus being potentially important for discovery of drugs against cancer. ER -