RT Journal Article
SR Electronic
T1 Circulating Long Non-coding RNAs in Plasma of Patients with Gastric Cancer
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3185
OP 3193
VO 33
IS 8
A1 TOMOHIRO ARITA
A1 DAISUKE ICHIKAWA
A1 HIROTAKA KONISHI
A1 SHUHEI KOMATSU
A1 ATSUSHI SHIOZAKI
A1 KATSUTOSHI SHODA
A1 TSUTOMU KAWAGUCHI
A1 SHOJI HIRAJIMA
A1 HIROAKI NAGATA
A1 TAKESHI KUBOTA
A1 HITOSHI FUJIWARA
A1 KAZUMA OKAMOTO
A1 EIGO OTSUJI
YR 2013
UL http://ar.iiarjournals.org/content/33/8/3185.abstract
AB Background: We examined the levels of long non-coding RNAs (lncRNAs) in the plasma of patients with gastric cancer to assess their clinical significance for diseases diagnosing and monitoring. Materials and Methods: We investigated the stability of plasma lncRNAs, and then confirmed the appropriateness of the lncRNA assay with a pre-amplification method. The levels of plasma lncRNAs, H19, HOX antisense intergenic RNA (HOTAIR), and metastasis associated lung adenocarcinoma transcript-1 (MALAT1), were then analyzed in patients with gastric cancer (GC) and healthy controls. Results: Plasma lncRNAs exhibited minimal gradual instability only under several severe conditions. Analysis showed that samples with pre-amplification had a higher level of linearity in the reverse transcription polymerase chain reaction (RT-PCR) assay than those without pre-amplification. Plasma H19 levels were significantly higher in patients than in healthy controls. Plasma H19 levels were significantly reduced in postoperative samples. Conclusion: Circulating lncRNAs can be detectable in plasma, and the detection of circulating lncRNAs may provide new complementary tumor markers for gastric cancer.