@article {WAZIR2185, author = {UMAR WAZIR and WEN G. JIANG and HEMAD YASAEI and HANNA LINNE and ROBERT F. NEWBOLD and KEFAH MOKBEL}, title = {P14ARF Is Down-regulated During Tumour Progression and Predicts the Clinical Outcome in Human Breast Cancer}, volume = {33}, number = {5}, pages = {2185--2189}, year = {2013}, publisher = {International Institute of Anticancer Research}, abstract = {The objective of this study was to determine the mRNA expression for p14 and p16 in a cohort of women with breast cancer. Materials and Methods: Breast cancer specimens (N= 127) and normal tissue (N=23) specimens were studied. Transcript levels were determined using quantitative polymerase chain reaction (PCR), and were correlated with clinicopathological data collected over 10 years. Results: Higher p14 mRNA transcript levels were associated with non-cancerous background tissue specimens (median copy numbers: 103 vs. 4, p=0.0095), with better overall and disease-free survival, and in TNM2 stage tumours (TNM2 vs. TNM1, 27.2 vs. 3.5, p=0.049; TNM1/TNM2 vs. TNM3/4, 26 vs. 2, p=0.009). There was no significant relationship between p16 levels and clinicopathological parameters. A correlation between p14 and human telomerase reverse transcriptase (hTERT) levels was observed (r=0.406, p=0.00005). Conclusion: p14 expression seems to increase initially in early breast cancer and decrease with further tumour progression. p14 may be induced to counteract immortalisation and hTERT surge.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/33/5/2185}, eprint = {https://ar.iiarjournals.org/content/33/5/2185.full.pdf}, journal = {Anticancer Research} }