RT Journal Article
SR Electronic
T1 A Color-coded Imaging Model of the Interaction of α<sub>v</sub> Integrin-GFP Expressed in Osteosarcoma Cells and RFP Expressing Blood Vessels in Gelfoam® Vascularized <em>In Vivo</em>
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1361
OP 1366
VO 33
IS 4
A1 UEHARA, FUMINARI
A1 TOME, YASUNORI
A1 YANO, SHUYA
A1 MIWA, SHINJI
A1 MII, SUMIYUKI
A1 HIROSHIMA, YUKIHIKO
A1 BOUVET, MICHAEL
A1 MAEHARA, HIROKI
A1 KANAYA, FUMINORI
A1 HOFFMAN, ROBERT M.
YR 2013
UL http://ar.iiarjournals.org/content/33/4/1361.abstract
AB The integrin family of proteins has been shown to be involved in the malignant behavior of cells. We report here development of a color-coded imaging model that can visualize the interaction between αv integrin linked to green fluorescent protein (GFP) in osteosarcoma cells and blood vessels in Gelfoam® vascularized after implantation in red fluorescent protein (RFP) transgenic nude mice. Human 143B osteosarcoma cells expressing αv integrin-GFP were generated by transfection with an αv integrin-GFP vector. Gelfoam® (5×5 mm) was transplanted subcutaneously in transgenic RFP nude mice. The implanted Gelfoam® became highly vascularized with RFP vessels within 14 days. Skin flaps were made at days 7, 14, 21, 28 after transplantation of Gelfoam® for observing vascularization of the Gelfoam® using fluorescence imaging. Gelfoam® is a useful tool to observe angiogenesis in vivo. 143B cells (5×105) expressing αv integrin-GFP were injected into the Gelfoam® seven days after transplantation of Gelfoam®. Seven days after cancer-cell injection, cancer cells and blood vessels were observed in the Gelfoam® by color-coded confocal microscopy via the skin flap. The 143B cells expressing αv integrin-GFP proliferated into the Gelfoam®, which contained RFP-expressing blood vessels. Strong expression of αv integrin-GFP in 143B cells was observed near RFP vessels in the Gelfoam®. The observation of the behavior of αv integrin-GFP and blood vessels will allow further understanding of the role of αv integrin in cancer cells.